The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development

• DOI: 10.1038/ncomms12119
• Publication Date: 2016-07-06T09:50:25Z
• Authors: Carsten Rautengarten; Berit Ebert; Lifeng Liu; Solomon Stonebloom; Andreia M. Smith-...; Markus Pauly; Ariel Orellana; Henrik Vibe Scheller; Joshua L. Heazlewood
• Keywords: Nucleotide sugar; Xyloglucan

Abstract Nucleotide sugar transport across Golgi membranes is essential for the luminal biosynthesis of glycan structures. Here we identify GDP-fucose transporter 1 (GFT1), an Arabidopsis nucleotide that translocates GDP- L -fucose into lumen. Using proteo-liposome-based assays, show GFT preferentially transports over other sugars in vitro , while GFT1 -silenced plants are almost devoid cell wall-derived xyloglucan and rhamnogalacturonan II. Furthermore, these lines display reduced content N -glycan structures accompanied by severe developmental growth defects. We conclude major import required proper plant development.