Structure of the cohesin loader Scc2

• DOI: 10.1038/ncomms13952
• Publication Date: 2017-01-06T12:06:16Z
• Authors: William C. H. Chao; Yasuto Murayama; Sofía Muñoz; Andrew W. Jones; Benjamin O. Wade; Andrew G. Purkiss; Xiao-Wen Hu; Aaron Borg; Ambrosius P. Snij...; Frank Uhlmann; Martin R. Singleton
• Keywords: subunidades de proteínas; Models, Molecular; 0301 basic medicine; subunidades de prote?nas; Saccharomyces cerevisiae Proteins; 24 Ciencias de la Vida; Chromosomal Proteins, Non-Histone; Science; prote?nas de Saccharomyces cerevisiae; Cell Cycle Proteins; Saccharomyces cerevisiae; Article; 03 medical and health sciences; Cohesins; Conserved Sequence; 0303 health sciences; Q; prote?nas del ciclo celular; secuencia conservada; accharomyces cerevisiae; uni?n proteica; Protein Subunits; proteínas de Saccharomyces cerevisiae; unión proteica; proteínas del ciclo celular; Protein Binding

Abstract The functions of cohesin are central to genome integrity, chromosome organization and transcription regulation through its prevention premature sister-chromatid separation the formation DNA loops. loading onto chromatin depends on Scc2–Scc4 complex; however, little is known about how it stimulates cohesion-loading activity. Here we determine large ‘hook’ structure Scc2 responsible for catalysing loading. We identify key surfaces that crucial in vivo . With aid previously determined structures homology modelling, derive a pseudo-atomic full-length complex. Finally, using recombinantly purified cohesin, performed crosslinking mass spectrometry interaction assays suggest uses modular make multiple contacts with cohesin.