Phylointeractomics reconstructs functional evolution of protein binding

• DOI: 10.1038/ncomms14334
• Publication Date: 2017-02-08T10:37:19Z
• Authors: Dennis Kappei; Marion Scheibe; Maciej Paszkowski...; Alina Bluhm; Toni Ingolf Gossmann; Sabrina Dietz; Mario Dejung; Holger Herlyn; Frank Buchholz; Matthias Mann; Falk Butter
• Keywords: Phylogenomics; Molecular evolution; Lineage (genetic)

Abstract Molecular phylogenomics investigates evolutionary relationships based on genomic data. However, despite sequence conservation, changes in protein interactions can occur relatively rapidly and may cause strong functional diversification. To investigate such evolution, we here combine with interaction proteomics. We develop this concept by investigating the molecular evolution of shelterin complex, which protects telomeres, across 16 vertebrate species from zebrafish to humans covering 450 million years evolution. Our phylointeractomics screen discovers previously unknown telomere-associated proteins reveals how homologous undergo For instance, show that TERF1 evolved as a telomere-binding common stem lineage marsupial placental mammals. Phylointeractomics is versatile scalable approach function thus provide experimental evidence for phylogenomic relationships.