Circadian deep sequencing reveals stress-response genes that adopt robust rhythmic expression during aging

• DOI: 10.1038/ncomms14529
• Publication Date: 2017-02-21T16:02:57Z
• Authors: Rachael C. Kuintzle; Eileen S. Chow; Tara N. Westby; Barbara O. Gvakharia; Jadwiga M. Giebul...; David A Hendrix
• Keywords: Piwi-interacting RNA

Abstract Disruption of the circadian clock, which directs rhythmic expression numerous output genes, accelerates aging. To enquire how system protects aging organisms, here we compare transcriptomes in heads young and old Drosophila melanogaster . The core clock most genes remained robustly flies, while others lost rhythmicity with age, resulting constitutive over- or under-expression. Unexpectedly, identify a subset that adopted increased de novo during aging, enriched for stress-response functions. These termed late-life cyclers, were also rhythmically induced flies by constant exposure to exogenous oxidative stress, this upregulation is CLOCK-dependent. We age-onset several putative primary piRNA transcripts overlapping antisense transposons. Our results suggest that, as organisms shifts greater regulatory priority mitigation accumulating cellular stress.