Regulation of hepatic lipogenesis by the zinc finger protein Zbtb20

Male 0301 basic medicine Science Carbohydrates Article 03 medical and health sciences Dietary Carbohydrates Animals Homeostasis Humans Cell Nucleus Mice, Knockout 0303 health sciences Basic Helix-Loop-Helix Leucine Zipper Transcription Factors Lipogenesis Q Nuclear Proteins 3. Good health Fatty Liver Mice, Inbred C57BL Protein Transport Glucose Gene Expression Regulation Liver Insulin Resistance Glycolysis Gene Deletion
DOI: 10.1038/ncomms14824 Publication Date: 2017-03-22T10:50:37Z
ABSTRACT
AbstractHepatic de novo lipogenesis (DNL) converts carbohydrates into triglycerides and is known to influence systemic lipid homoeostasis. Here, we demonstrate that the zinc finger protein Zbtb20 is required for DNL. Mice lacking Zbtb20 in the liver exhibit hypolipidemia and reduced levels of liver triglycerides, along with impaired hepatic lipogenesis. The expression of genes involved in glycolysis and DNL, including that of two ChREBP isoforms, is decreased in livers of knockout mice. Zbtb20 binds to and enhances the activity of the ChREBP-α promoter, suggesting that altered metabolic gene expression is mainly driven by ChREBP. In addition, ChREBP-β overexpression largely restores hepatic expression of genes involved in glucose and lipid metabolism, and increases plasma and liver triglyceride levels in knockout mice. Finally, we show that Zbtb20 ablation protects from diet-induced liver steatosis and improves hepatic insulin resistance. We suggest ZBTB20 is an essential regulator of hepatic lipogenesis and may be a therapeutic target for the treatment of fatty liver disease.
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