A damaged genome’s transcriptional landscape through multilayered expression profiling around in situ-mapped DNA double-strand breaks

• DOI: 10.1038/ncomms15656
• Publication Date: 2017-05-31T12:12:53Z
• Authors: Fabio Iannelli; Alessandro Galbiati; Ilaria Capozzo; Quan Nguyen; Brian Magnuson; Flavia Michelini; Giuseppina D’Ales...; Matteo Cabrini; Marco Roncador; Sofia Francia; Nicola Crosetto; Mats Ljungman; Piero Carninci; Fabrizio d’Adda d...
• Keywords: Transcription; RNA polymerase II

Abstract Of the many types of DNA damage, double-strand breaks (DSBs) are probably most deleterious. Mounting evidence points to an intricate relationship between DSBs and transcription. A cell system in which impact on transcription can be investigated at precisely mapped genomic is essential study this relationship. Here a human line, we map genome-wide high resolution induced by restriction enzyme, characterize their gene expression four independent approaches monitoring steady-state RNA levels, rates synthesis, initiation polymerase II elongation. We consistently observe transcriptional repression proximity DSBs. Downregulation depends ATM kinase activity distance from DSB. Our couples for first time, best our knowledge, high-resolution mapping with multilayered transcriptomics dissect events shaping after DSB induction multiple endogenous sites.