Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica

• DOI: 10.1038/ncomms3156
• Publication Date: 2013-07-23T13:21:05Z
• Authors: Michael Kube; Tatyana N. Cherni...; Yamal Al-Ramahi; Ana Beloqui; Nieves Lopez-Cortez; María-Eugenia Gua...; Hermann J. Heipieper; Sven Klages; Oleg R. Kotsyurbenko; Ines Langer; Taras Y. Nechitaylo; Heinrich Lünsdorf; Marisol Fernández; Silvia Juárez; Sergio Ciordia; Alexander Singer; Olga Kagan; Olga Egorova; Pierre Alain Petit; Peter Stogios; Youngchang Kim; Anatoli Tchigvintsev; Robert Flick; Renata Denaro; Maria Genovese; Juan P. Albar; Oleg N. Reva; Montserrat Martín...; Hai Tran; Manuel Ferrer; Alexei Savchenko; Alexander F. Yakunin; Michail M. Yakimov; Olga V. Golyshina; Richard Reinhardt; Peter N. Golyshin
• Keywords: Mesophile

Ubiquitous bacteria from the genus Oleispira drive oil degradation in largest environment on Earth, cold and deep sea. Here we report genome sequence of antarctica show that compared with Alcanivorax borkumensis—the paradigm mesophilic hydrocarbonoclastic bacteria—O. has a larger witnessed massive gene-transfer events. We identify an array alkane monooxygenases, osmoprotectants, siderophores micronutrient-scavenging pathways. also at low temperatures, main protein-folding machine Cpn60 functions as single heptameric barrel uses proteins substrates classical double-barrel structure observed higher temperatures. With 11 protein crystal structures, further set structures one psychrotolerant organism. The most common structural feature is increased content surface-exposed negatively charged residues to their counterparts. Our findings are relevant context microbial cold-adaptation mechanisms development strategies for oil-spill mitigation environments. oil-degrading bacterium found Kube et al. O. antarcticaand provide comprehensive functional genetic analysis, revealing insights into how this organism adapted its environment.