Apical membrane antigen 1 mediates apicomplexan parasite attachment but is dispensable for host cell invasion

• DOI: 10.1038/ncomms3552
• Publication Date: 2013-10-10T10:42:05Z
• Authors: Daniel Y. Bargieri; Nicole Andenmatten; Vanessa Lagal; Sabine Thiberge; Jamie A. Whitelaw; Isabelle Tardieux; Markus Meissner; Robert Ménard
• Keywords: 0301 basic medicine; Plasmodium berghei; Molecular Sequence Data; Protozoan Proteins; Gene Expression; Membrane Proteins; Antigens, Protozoan; Article; Host-Parasite Interactions; Malaria; Rats; 3. Good health; Mice; 03 medical and health sciences; Animals; Female; Amino Acid Sequence; Rats, Wistar; Toxoplasma; Conserved Sequence; Gene Deletion; Toxoplasmosis; Protein Binding

Apicomplexan parasites invade host cells by forming a ring-like junction with the cell surface and actively sliding through the junction inside an intracellular vacuole. Apical membrane antigen 1 is conserved in apicomplexans and a long-standing malaria vaccine candidate. It is considered to have multiple important roles during host cell penetration, primarily in structuring the junction by interacting with the rhoptry neck 2 protein and transducing the force generated by the parasite motor during internalization. Here, we generate Plasmodium sporozoites and merozoites and Toxoplasma tachyzoites lacking apical membrane antigen 1, and find that the latter two are impaired in host cell attachment but the three display normal host cell penetration through the junction. Therefore, apical membrane antigen 1, rather than an essential invasin, is a dispensable adhesin of apicomplexan zoites. These genetic data have implications on the use of apical membrane antigen 1 or the apical membrane antigen 1-rhoptry neck 2 interaction as targets of intervention strategies against malaria or other diseases caused by apicomplexans.