Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors

• DOI: 10.1038/ncomms7500
• Publication Date: 2015-03-23T12:59:56Z
• Authors: Reuven Edri; Yakey Yaffe; Michael J. Ziller; Naresh Mutukula; Rotem Volkman; Eyal David; Jasmine Jacob-Hirsch; Hagar Malcov; Carmit Levy; Gideon Rechavi; Irit Gat-Viks; Alexander Meissner; Yechiel Elkabetz
• Keywords: Neuroepithelial cell; Progenitor; Neural Development

Abstract Decoding heterogeneity of pluripotent stem cell (PSC)-derived neural progeny is fundamental for revealing the origin diverse progenitors, defining their lineages, and identifying fate determinants driving transition through distinct potencies. Here we have prospectively isolated consecutively appearing PSC-derived primary progenitors based on Notch activation state. We first isolate early neuroepithelial cells show broad Notch-dependent developmental proliferative potential. Neuroepithelial further yield successive functional from midneurogenic radial glia derived basal to gliogenic adult-like together recapitulating hallmarks (NSC) ontogeny. Gene expression profiling reveals dynamic stage-specific transcriptional patterns that may link development progenitor identities activation. Our observations provide a platform characterization manipulation types amenable developing streamlined lineage specification paradigms modelling in health disease.