A Time-Encoded Technique for fibre-based hyperspectral broadband stimulated Raman microscopy

• DOI: 10.1038/ncomms7784
• Publication Date: 2015-04-17T11:20:24Z
• Authors: Sebastian Karpf; Matthias Eibl; Wolfgang Wieser; Thomas Klein; Robert Huber
• Keywords: Solutions; Time Factors; Fourier Analysis; Plant Stems; Cyclohexanes; Geranium; Benzene; Spectrum Analysis, Raman; Article; Toluene

AbstractRaman sensing and microscopy are among the most specific optical technologies to identify the chemical compounds of unknown samples, and to enable label-free biomedical imaging. Here we present a method for stimulated Raman scattering spectroscopy and imaging with a time-encoded (TICO) Raman concept. We use continuous wave, rapidly wavelength-swept probe lasers and combine them with a short-duty-cycle actively modulated pump laser. Hence, we achieve high stimulated Raman gain signal levels, while still benefitting from the narrow linewidth and low noise of continuous wave operation. Our all-fibre TICO-Raman setup uses a Fourier domain mode-locked laser source to achieve a unique combination of high speed, broad spectral coverage (750–3,150 cm−1) and high resolution (0.5 cm−1). The Raman information is directly encoded and acquired in time. We demonstrate quantitative chemical analysis of a solvent mixture and hyperspectral Raman microscopy with molecular contrast of plant cells.