Artificial membrane-binding proteins stimulate oxygenation of stem cells during engineering of large cartilage tissue

• DOI: 10.1038/ncomms8405
• Publication Date: 2015-06-17T10:35:20Z
• Authors: James P. K. Armst...; Rameen Shakur; Joseph P. Horne; Sally C. Dickinson; Craig T. Armstrong; Katherine Lau; Juned Kadiwala; Robert Lowe; Annela Seddon; Stephen Mann; J. L. Ross Anderson; Adam W. Perriman; Anthony P. Hollander
• Keywords: Surface Modification; Surface Engineering

Restricted oxygen diffusion can result in central cell necrosis engineered tissue, a problem that is exacerbated when engineering large tissue constructs for clinical application. Here we show pre-treating human mesenchymal stem cells (hMSCs) with synthetic membrane-active myoglobin-polymer-surfactant complexes provide reservoir of capable alleviating at the centre hyaline cartilage. This achieved through development new functionalization methodology based on polymer-surfactant conjugation, which allows delivery functional proteins to hMSC membrane. approach circumvents need surface using protein chimerization or genetic transfection, and demonstrate surface-modified hMSCs retain their ability proliferate undergo multilineage differentiation. The technology facile, versatile non-disruptive, addition oxygenation, it should have far-reaching application host cell-based therapies.