Structure and gating of the nuclear pore complex

• DOI: 10.1038/ncomms8532
• Publication Date: 2015-06-26T11:42:14Z
• Authors: Matthias Eibauer; Mauro Pellanda; Yagmur Turgay; Anna Dubrovsky; Annik Wild; Ohad Medalia
• Keywords: Nuclear pore; Nucleoporin; NLS

Abstract Nuclear pore complexes (NPCs) perforate the nuclear envelope and allow exchange of macromolecules between nucleus cytoplasm. To acquire a deeper understanding this transport mechanism, we analyse structure NPC scaffold permeability barrier, by reconstructing Xenopus laevis oocyte from native envelopes up to 20 Å resolution cryo-electron tomography in conjunction with subtomogram averaging. In addition resolving individual protein domains constituents, propose model for architecture molecular gate at its central channel. Furthermore, compare contrast one that exhibits reduced activity unveil spatial properties gate.