Simultaneous generation of many RNA-seq libraries in a single reaction

• DOI: 10.1038/nmeth.3313
• Publication Date: 2015-03-02T16:17:42Z
• Authors: Alexander A Shishkin; Georgia Giannoukos; Alper Kucukural; Dawn Ciulla; Michele Busby; Christine Surka; Jenny Chen; Roby P Bhattacharyya; Robert F Rudy; Milesh M Patel; Nathaniel Novod; Deborah T Hung; Andreas Gnirke; Manuel Garber; Mitchell Guttman; Jonathan Livny
• Keywords: *Gene Library; 0301 basic medicine; Time Factors; Bacteria; Base Sequence; Bioinformatics; Sequence Analysis, RNA; Integrative Biology; Systems Biology; Gene Expression Profiling; Biophysics; Computational Biology; Biochemistry; 03 medical and health sciences; *Base Sequence; and Structural Biology; RNA; Sequence Analysis; Gene Library

Although RNA-seq is a powerful tool, the considerable time and cost associated with library construction has limited its utilization for various applications. RNAtag-Seq, an approach to generate multiple RNA-seq libraries in a single reaction, lowers time and cost per sample, and it produces data on prokaryotic and eukaryotic samples that are comparable to those generated by traditional strand-specific RNA-seq approaches.