Adaptive optics improves multiphoton super-resolution imaging

• DOI: 10.1038/nmeth.4337
• Publication Date: 2017-06-19T15:01:48Z
• Authors: Wei Zheng; Yicong Wu; Peter Winter; Robert Fischer; Damian Dalle Nogare; Amy Hong; Chad McCormick; Ryan Christensen; William P Dempsey; Don B Arnold; Joshua Zimmerberg; Ajay Chitnis; James Sellers; Clare Waterman; Hari Shroff
• Keywords: Two-photon excitation microscopy

We improve multiphoton structured illumination microscopy using a nonlinear guide star to determine optical aberrations and a deformable mirror to correct them. We demonstrate our method on bead phantoms, cells in collagen gels, nematode larvae and embryos, Drosophila brain, and zebrafish embryos. Peak intensity is increased (up to 40-fold) and resolution recovered (up to 176 ± 10 nm laterally, 729 ± 39 nm axially) at depths ∼250 μm from the coverslip surface.