A common origin of synaptic vesicles undergoing evoked and spontaneous fusion

• DOI: 10.1038/nn.2695
• Publication Date: 2010-11-21T19:21:21Z
• Authors: Yunfeng Hua; Raunak Sinha; Magalie Martineau; Martin Kahms; Jürgen Klingauf
• Keywords: 0301 basic medicine; 03 medical and health sciences; Action Potentials; Animals; Synaptic Vesicles; Rats, Wistar; Evoked Potentials; Hippocampus; Membrane Fusion; Synaptic Transmission; Endocytosis; Rats

There is a longstanding controversy on the identity of synaptic vesicles undergoing spontaneous versus evoked release. A recent study, introducing a new genetic probe, suggested that spontaneous release is driven by a resting pool of synaptic vesicles refractory to stimulation. We found that cross-depletion of spontaneously or actively recycling synaptic vesicle pools occurred on stimulation in rat hippocampal neurons and identified the recycling pool as a major source of spontaneous release.