Enhanced protection conferred by mucosal BCG vaccination associates with presence of antigen-specific lung tissue-resident PD-1+ KLRG1− CD4+ T cells

• DOI: 10.1038/s41385-018-0109-1
• Publication Date: 2018-11-16T10:16:17Z
• Authors: N.C. Bull; E. Stylianou; D.A. Kaveh; N. Pinpathomrat; J. Pasricha; R. Harrington-Kandt; M.C. Garcia-Pelayo; P.J. Hogarth; H. McShane
• Keywords: Parenchyma

BCG, the only vaccine licensed against tuberculosis, demonstrates variable efficacy in humans. Recent preclinical studies highlight potential for mucosal BCG vaccination to improve protection. Lung tissue-resident memory T cells reside within parenchyma, potentially playing an important role protective immunity tuberculosis. We hypothesised that may enhance generation of lung cells, affording improved protection Mycobacterium In a mouse model, intranasal (IN) conferred superior lungs compared systemic intradermal (ID) route. Intravascular staining allowed discrimination CD4+ from those vasculature, revealing resulted increased frequency antigen-specific vaccination. Tissue-resident induced by displayed enhanced proliferative capacity vascular and splenic cells. Only expressing PD-1+ KLRG1- cell-surface phenotype. These constitute BCG-induced population which be responsible observed with IN demonstrate significantly improves over this correlates novel