Epithelial GPR35 protects from Citrobacter rodentium infection by preserving goblet cells and mucosal barrier integrity

• DOI: 10.1038/s41385-022-00494-y
• Publication Date: 2022-03-09T08:02:31Z
• Authors: Hassan Melhem; Berna Kaya; Tanay Kaymak; Philipp Wuggenig; Emilio Flint; Julien Roux; Koen C. Oost; Claudia Cavelti-W...; Maria L. Balmer; Jean-Claude Walser; Rodrigo A. Morales; Christian U. Riedel; Prisca Liberali; Eduardo J. Villab...; Jan Hendrik Niess
• Keywords: Citrobacter rodentium; Goblet cell; Barrier function; Pyroptosis

Goblet cells secrete mucin to create a protective mucus layer against invasive bacterial infection and are therefore essential for maintaining intestinal health. However, the molecular pathways that regulate goblet cell function remain largely unknown. Although GPR35 is highly expressed in colonic epithelial cells, its importance promoting barrier unclear. In this study, we show Gpr35 plays critical role function. mice, cell-type-specific deletion of but not macrophages results depletion dysbiosis, rendering these animals more susceptible Citrobacter rodentium infection. Mechanistically, scRNA-seq analysis indicates signaling maintain normal pyroptosis levels cells. Our work shows presence element cell-mediated symbiosis between host microbiota. through their production secretion mucins play an protecting luminal insults, contribute mutualism gut Loss disrupts compartment by reducing numbers increasing levels. Dysregulation correlated with changes mucosa-associated communities. As result, Gpr35f/fVil+ mice displayed increased susceptibility C. rodentium-induced colitis. (Adapted from SERVIER MEDICAL ART (CC license 3.0)).