Altered gut microbiome composition by appendectomy contributes to colorectal cancer

• DOI: 10.1038/s41388-022-02569-3
• Publication Date: 2022-12-20T06:02:58Z
• Authors: Feiyu Shi; Gaixia Liu; Yufeng Lin; Cosmos liutao Guo; Jing Han; Eagle S. H. Chu; Chengxin Shi; Yaguang Li; Haowei Zhang; Chenhao Hu; Ruihan Liu; Shuixiang He; Gang Guo; Yinnan Chen; Xiang Zhang; Olabisi Oluwabuko...; Sunny Hei Wong; Jun Yu; Junjun She
• Keywords: Prevotella; Dysbiosis; Bacteroides fragilis; Fusobacterium; Veillonella; Lachnospiraceae

Abstract Appendectomy impacts the homeostasis of gut microbiome in patients. We aimed to study role appendectomy colorectal cancer (CRC) risk through causing microbial dysbiosis. Population-based longitudinal (cohort 1, n = 129,155) showed a 73.0% increase CRC among cases throughout 20 years follow-up (Adjusted sub-distribution hazard ratio (SHR) 1.73, 95% CI 1.49–2.01, P < 0.001). Shotgun metagenomic sequencing was performed on fecal samples from cohort 2 ( 314). Gut dysbiosis subjects observed with significant enrichment 7 CRC-promoting bacteria Bacteroides vulgatus, fragilis, Veillonella dispar, Prevotella ruminicola, fucsa, dentalis, denticola ) and depletion 5 beneficial commensals Blautia sp YL58, Enterococcus hirae, Lachnospiraceae bacterium Choco86, Collinsella aerofaciens, SC05B48 ). Microbial network analysis increased correlation strengths enriched their oncogenic pathways compared controls. Of which, B. fragilis centrality bacteria. further confirmed that promoted tumorigenesis mice by impaired intestinal barrier function. Collectively, this revealed appendectomy-induced characterized depleted commensals, signifying may play crucial development induced appendectomy.