Exosomal miR-21-5p derived from gastric cancer promotes peritoneal metastasis via mesothelial-to-mesenchymal transition

• DOI: 10.1038/s41419-018-0928-8
• Publication Date: 2018-08-28T17:22:15Z
• Authors: Qiang Li; Bowen Li; Qing Li; Song Wei; Zhongyuan He; Xiaoxu Huang; Lu Wang; Yiwen Xia; Zhipeng Xu; Zheng Li; Weizhi Wang; Li Yang; Diancai Zhang; Zekuan Xu
• Keywords: Gentamicin protection assay; Exosome

Abstract Peritoneal metastasis is a primary metastatic route for gastric cancers, and the mechanisms underlying this process are still unclear. mesothelial cells (PMCs) undergo mesothelial-to-mesenchymal transition (MMT) to provide favorable environment cancer cells. In study, we investigated how exosomal miR-21-5p induces MMT promotes peritoneal metastasis. Gastric (GC)-derived exosomes were identified by transmission electron microscopy western blot analysis, then uptake of was confirmed PKH-67 staining. The expression SMAD7 measured quantitative real-time polymerase chain reaction (qRT-PCR) blot, interactions between its target genes Luciferase reporter assays. PMCs determined invasion assays, adhesion immunofluorescent assay, blot. Meanwhile, mouse model tumor dissemination performed investigate role in vivo. We found that could internalize GC-derived led increased levels PMCs. Through various types vitro vivo able induce promote Moreover, our study revealed promoted through activating TGF-β/Smad pathway via targeting SMAD7. Altogether, data suggest may be novel therapeutic GC