Long noncoding RNA PCAT1, a novel serum-based biomarker, enhances cell growth by sponging miR-326 in oesophageal squamous cell carcinoma

• DOI: 10.1038/s41419-019-1745-4
• Publication Date: 2019-07-04T13:41:58Z
• Authors: Lijie Huang; Yan Wang; Jiao Chen; Yu Wang; Yabing Zhao; Yali Wang; Yunping Ma; Xin Chen; Wenzhong Liu; Zhengzheng Li; Lianmei Zhao; Baoen Shan; Xin Dong; Dan Li; Shujuan Shao; Yongmei Song; Qimin Zhan; Xuefeng Liu
• Keywords: Male; 0301 basic medicine; Base Sequence; Carcinogenesis; Mice, Nude; Exosomes; Article; Up-Regulation; 3. Good health; Gene Expression Regulation, Neoplastic; MicroRNAs; 03 medical and health sciences; Cell Line, Tumor; Biomarkers, Tumor; Animals; Humans; RNA, Long Noncoding; Esophageal Squamous Cell Carcinoma; Cell Proliferation

Long noncoding RNAs (lncRNAs) play important roles in the development and progression of human cancers. The lncRNA prostate cancer-associated transcript 1 (PCAT1) has been reported to be involved multiple cancers, including oesophageal squamous cell carcinoma (ESCC). However, detailed biological functions, underlying mechanisms clinical relevance PCAT1 ESCC remain unclear. Here, we confirmed that was highly expressed tissues lines. Knockdown inhibited growth cells, whereas overexpression showed opposite effect both vitro vivo. Moreover, knockdown arrested cycle at G2/M phase, reduced expression cyclin B1 CDC2, caused cells more sensitive paclitaxel. Furthermore, could bind miR-326, a tumour suppressor diverse Rescue experiments revealed enforced miR-326 attenuated promotive on growth. In addition, discovered present cell-derived exosomes, higher serum patients than those healthy volunteer donors, promoted through exosomes. Thus, our data indicate promotes proliferation by sponging may serve as non-invasive biomarker for ESCC.