The novel circCLK3/miR-320a/FoxM1 axis promotes cervical cancer progression

• DOI: 10.1038/s41419-019-2183-z
• Publication Date: 2019-12-12T15:10:09Z
• Authors: Hanqing Hong; Hai Zhu; Shujun Zhao; Kaili Wang; Nan Zhang; Yun Tian; Yan Li; Yaping Wang; Xiaofeng Lv; Tianxiang Wei; Yan Liu; Suzhen Fan; Yang Liu; Yuan Li; Aojie Cai; Shuo Jin; Qiaohong Qin; Hongyu Li
• Keywords: FOXM1

Abstract As a new class of non-coding RNA, circular RNAs (circRNAs) play crucial roles in the development and progression various cancers. However, detailed functions circRNAs cervical cancer have seldom been reported. In this study, circRNA sequence was applied to detect differentially expressed between tissues adjacent normal tissues. The relationships circCLK3 level with clinicopathological characteristics prognosis were analyzed. vitro CCK-8, cell count, colony, wound healing, transwell migration invasion, vivo tumorigenesis lung metastasis models performed evaluate circCLK3. pull-down, RNA immunoprecipitation (RIP), luciferase reporter rescue assays employed clarify interaction miR-320a regulation on FoxM1. We found that remarkably higher than tissues, closely associated tumor differentiation, FIGO stage depth stromal invasion. Down-regulated evidently inhibited growth vivo, while up-regulated significantly promoted vivo. RIP demonstrated directly bound sponge miR-320a. MiR-320a suppressed expression FoxM1 through binding 3′UTR mRNA. addition, proliferation, migration, invasion cancer, suppressing FoxM1, enhanced sponging promoting expression. summary, may serve as novel diagnostic biomarker for disease promising molecular target early diagnoses treatments cancer.