SOX9-activated FARSA-AS1 predetermines cell growth, stemness, and metastasis in colorectal cancer through upregulating FARSA and SOX9

• DOI: 10.1038/s41419-020-03273-4
• Publication Date: 2020-12-14T18:03:44Z
• Authors: Taicheng Zhou; Lili Wu; Ning Ma; Fuxin Tang; Zhuomin Yu; Zhipeng Jiang; Yingru Li; Zhen Zong; Kunpeng Hu
• Keywords: SOX9

Abstract SRY-box transcription factors (SOXs) are effective inducers for the formation of stem-like phenotypes. As a member SOX family, SOX9 (SRY-box factor 9) has been reported to be highly expressed and exert oncogenic functions in multiple human cancers. In this study, we hypothesized that could regulate function cancer stem/initiating cells (CSCs) further facilitate progression colorectal (CRC). Then, stable transfection shRNAs was used silence indicated genes. Loss-of-function experiments were conducted demonstrate vitro CRC cells. vivo study determine changes tumorigenesis metastasis vivo. Bioinformatics analyses mechanistic employed explore downstream molecules. Presently, GEPIA data upregulated 275 COAD (colon adenocarcinoma) samples relative 349 normal tissues. Besides, also proved upregulation cell lines (HCT15, SW480, SW1116, HT-29) compared NCM-460 Silencing suppressed growth, stemness, migration, invasion. Mechanistically, activated lncRNA phenylalanyl-tRNA synthetase subunit alpha antisense RNA 1 ( FARSA-AS1 ), while elevated turn by absorbing miR-18b-5p augmented FARSA via sequestering miR-28-5p . Furthermore, loss hindered malignant phenotypes blocked tumor growth Notably, testified aggravated malignancy enhancing Our unveiled mechanism SOX9- - / loop CRC, which provides some clews promising targets CRC.