CircSPI1 acts as an oncogene in acute myeloid leukemia through antagonizing SPI1 and interacting with microRNAs

• DOI: 10.1038/s41419-021-03566-2
• Publication Date: 2021-03-19T10:14:54Z
• Authors: Xiaoling Wang; Peng Jin; Yi Zhang; Kankan Wang
• Keywords: Leukemia, Myeloid, Acute; MicroRNAs; 0303 health sciences; 03 medical and health sciences; QH573-671; Proto-Oncogene Proteins; Trans-Activators; Humans; Oncogenes; RNA, Circular; Cytology; Article

Abstract PU.1 (encoded by SPI1 ) is essential for myeloid development, and inhibition of its expression activity can lead to acute leukemia (AML). The precise regulation crucial the development AML, discovery circular RNAs (circRNAs) add a new layer information on regulation. Here, we found that circSPI1, RNA derived from gene, highly expressed in AML but not normal counterparts. Unlike SPI1, tumor suppressor being lowly demonstrate circSPI1 acts as an oncogene, evidenced observation knockdown induces differentiation apoptosis cells. We provide mechanistic evidence multiple regulatory roles progression. On one hand, contributes cells interacting with translation initiation factor eIF4AIII antagonize at level. other proliferation miR-1307-3p, miR-382-5p, miR-767-5p; this role uncoupled SPI1. Finally, illustrate clinical significance showing circSPI1-regulated genes are associated outcome patients. Our data insight into complex gene now involving circSPI1.