METTL3-mediated mRNA N6-methyladenosine is required for oocyte and follicle development in mice

• DOI: 10.1038/s41419-021-04272-9
• Publication Date: 2021-10-23T10:02:43Z
• Authors: Haiyuan Mu; Ting Zhang; Ying Yang; Danru Zhang; Jie Gao; Junhong Li; Liang Yue; Dengfeng Gao; Bingbo Shi; Yue Han; Liang Zhong; Xinze Chen; Zhen-Bo Wang; Zhen Lin; Ming-Han Tong; Qing-Yuan Sun; Yun-Gui Yang; Jianyong Han
• Keywords: N6-Methyladenosine

Abstract Proper follicle development is very important for the production of mature oocytes, which essential maintenance female fertility. This complex biological process requires precise gene regulation. The most abundant modification mRNA, N 6 -methyladenosine (m A), involved in many RNA metabolism processes, including splicing, translation, stability, and degradation. Here, we report that m A plays roles during oocyte development. Oocyte-specific inactivation key methyltransferase Mettl3 with Gdf9 -Cre caused DNA damage accumulation defective development, abnormal ovulation. Mechanistically, combined RNA-seq methylated immunoprecipitation sequencing (MeRIP-seq) data from oocytes revealed, found METTL3 targets Itsn2 then enhances its stability to influence meiosis. Taken together, our findings highlight crucial mRNA coordination stabilization growth.