LncRNA RP11-89 facilitates tumorigenesis and ferroptosis resistance through PROM2-activated iron export by sponging miR-129-5p in bladder cancer

• DOI: 10.1038/s41419-021-04296-1
• Publication Date: 2021-11-02T08:02:48Z
• Authors: Wenjie Luo; Jun Wang; Wenhao Xu; Chunguang Ma; Fangning Wan; Yongqiang Huang; Mengfei Yao; Hailiang Zhang; Yuanyuan Qu; Dingwei Ye; Yiping Zhu
• Keywords: Male; Carcinogenesis; Iron; Mice, Nude; Article; Cohort Studies; 03 medical and health sciences; Cell Line, Tumor; Animals; Ferroptosis; Humans; Aged; Mice, Inbred BALB C; 0303 health sciences; Binding Sites; Membrane Glycoproteins; QH573-671; Base Sequence; Biological Transport; Cell Cycle Checkpoints; 3. Good health; Gene Expression Regulation, Neoplastic; MicroRNAs; Ferritins; Female; Cytology

Abstract Long non-coding RNAs (lncRNAs) act as important regulators of tumorigenesis and development in bladder cancer. However, the underlying molecular mechanisms remain elusive. We previously identified a novel lncRNA signature related to immunity progression Here we further explored function RP11-89, discovered previous signature. Loss- gain-of experiments were performed using CCK-8 assay, flow cytometry, Transwell assays, scratch tests subcutaneous nude mouse models. High-throughput RNA sequencing was conducted identify dysregulated genes cancer cells with RP11-89 knockdown or overexpression. Regulation on miR-129-5p PROM2 through luciferase reporter RIP assay pull-down assay. promoted cell proliferation, migration inhibited cycle arrest via miR-129-5p/PROM2 axis. found that “sponges” upregulates PROM2. Elevated associated attenuated ferroptosis iron export, formation multivesicular bodies less mitochondrial abnormalities. demonstrated is tumorigenic regulator inhibits PROM2-activated export. may serve potential biomarker for targeted therapy