LncRNA HOXC-AS3 promotes non-small-cell lung cancer growth and metastasis through upregulation of YBX1

• DOI: 10.1038/s41419-022-04723-x
• Publication Date: 2022-04-06T06:09:28Z
• Authors: HongBo Su; GuanZhi Fan; Jin Huang; XueShan Qiu
• Keywords: Chromatin immunoprecipitation; Immunoprecipitation

Abstract NSCLC is common and the primary cause of cancer-related deaths due to a lack early diagnosis its propensity for metastasis. The pathogenesis still unclear. Here, we explored molecular mechanisms underlying development, focusing on HOXC-AS3/YBX1/HOXC8 axis. Human specimens cell lines were used. qRT-PCR western blotting utilised examine levels HOXC-AS3/YBX1/HOXC8. CCK-8, colony formation, scratch wound healing Transwell assays performed evaluate cancer proliferation, migration invasion. A nude mouse xenograft model was used tumour growth metastasis in vivo. RNA pull-down, chromatin immunoprecipitation, coimmunoprecipitation dual-luciferase applied validate interactions HOXC-AS3/YBX1, MDM2/YBX1 YBX1/HOXC8 promoter. HOXC-AS3 HOXC8 increased human cells. Knockdown suppressed invasion, as well directly bound YBX1 suppress ubiquitination mediated by MDM2. promoter enhanced transcription. inhibited effects overexpression NSCLC. promotes stabilising thus increasing Our study indicates that axis could serve biomarker or target therapy development.