IGF2BP3 enhances lipid metabolism in cervical cancer by upregulating the expression of SCD

• DOI: 10.1038/s41419-024-06520-0
• Publication Date: 2024-02-14T07:02:24Z
• Authors: Chenying Han; Chenchen Hu; Tianyue Liu; Yuanjie Sun; Feiming Hu; Yuanli He; Jiaxing Zhang; Jiaxi Chen; Jiaqi Ding; Jiangjiang Fan; Xiyang Zhang; Jing Wang; Xupeng Qiao; Dongbo Jiang; Kun Yang; Shuya Yang
• Keywords: 0301 basic medicine; 0303 health sciences; QH573-671; Cytology; Article

Abstract Cervical cancer (CC) is the most common gynecologic malignancy, which seriously threatens health of women. Lipid metabolism necessary for tumor proliferation and metastasis. However, molecular mechanism relationship between CC lipid remains poorly defined. We revealed expression IGF2BP3 in exceeded adjacent tissues, was positively associated with stage using human tissue microarrays. The Cell Counting Kit-8, colony formation assay, 5-ethynyl-2′-deoxyuridine transwell assays, wound-healing flow cytometry assessed role metastasis cells. Besides, exploring participating IGF2BP3-driven used RNA-seq, determined SCD as target IGF2BP3. Further, droplets, cellular triglyceride (TG) contents, fatty acids were accessed to discover that can enhance CC. Moreover, RIP assay methylated RNA immunoprecipitation experiments seeked aimed-gene-binding specificity. Lastly, knockdown restrained growth metabolism, after overexpression rescued influence vitro vivo nude mouse tumor-bearing model. Mechanistically, regulated mRNA m6A modifications via IGF2BP3-METTL14 complex, thereby enhanced proliferation, metastasis, metabolism. Our study highlights plays a crucial progression represents therapeutic latent strategy. It potential tactic blocks metabolic pathway relevant purpose treating