Microarray profiling and co-expression network analysis of lncRNAs and mRNAs in ovarian cancer

• DOI: 10.1038/s41420-019-0173-7
• Publication Date: 2019-05-09T00:42:03Z
• Authors: Ce Gao; Di Zhao; Qing Zhao; Dandan Dong; Lin Mu; Xuejun Zhao; Man Guo; Aili Xu; Lei Fang; Qian Liu; Jianhua Che
• Keywords: Viability assay

Dysregulated long noncoding RNAs (lncRNAs) are involved in the pathogenesis and development of human diseases, such as epithelial ovarian cancer (EOC). In this study, we identified EOC-related lncRNAs performed lncRNA mRNA microarray analyses using IOSE80, a normal ovary cell line, two carcinoma lines (SKOV3 SKOV3/DDP) to investigate potential roles EOC. lncRNA-HEIH expression EOC tissues was measured by quantitative real-time polymerase chain reaction (qPCR). addition, generated lncRNA-mRNA co-expression network order identify lncRNA-expression trends target genes. Cell viability, migration, invasion were determined Counting Kit-8, transwell assay, wound-healing respectively, apoptosis analyzed flow cytometry. We 3527 differentially expressed upon comparison profiles from IOSE80 with those SKOV3 lines, 11 confirmed qPCR. Both pathway gene ontology demonstrated involvement lncRNAs, especially HEIH LINC-PINT, multiple biological processes. Furthermore, vitro knockdown experiments that suppression inhibited proliferation. Our findings provide foundation for further research into role these carcinogenesis progression.