Use of a dual genetic system to decipher exocrine cell fate conversions in the adult pancreas

• DOI: 10.1038/s41421-022-00485-0
• Publication Date: 2023-01-03T11:04:01Z
• Authors: Huan Zhao; Xiuzhen Huang; Zixin Liu; Liang Lai; Ruilin Sun; Ruling Shen; Yan Li; Lingjuan He; Wenjuan Pu; Zan Lv; Yi Li; Ximeng Han; Xiuxiu Liu; Bin Zhou
• Keywords: Transdifferentiation; Ductal cells; Acinar cell; PDX1; Neogenesis

Unraveling cell fate plasticity during tissue homeostasis and repair can reveal actionable insights for stem biology regenerative medicine. In the pancreas, it remains controversial whether lineage transdifferentiation among exocrine cells occur under pathophysiological conditions. Here, to address this question, we used a dual recombinase-mediated genetic system that enables simultaneous tracing of pancreatic acinar ductal using two distinct reporters, avoiding "ectopic" labeling by Cre-loxP recombination system. We found acinar-to-ductal occurs after duct ligation or caerulein-induced pancreatitis, but not partial pancreatectomy. On other hand, contribute new significant loss. By proliferation, also quantify proliferation dynamics deduce turnover rate lineages homeostasis. Together, these results suggest happens between in glands specific