Complete genome analysis demonstrates multiple introductions of enterovirus 71 and coxsackievirus A16 recombinant strains into Thailand during the past decade

• DOI: 10.1038/s41426-018-0215-x
• Publication Date: 2018-12-14T05:10:27Z
• Authors: Pirom Noisumdaeng; Kantima Sangsiriwut; Jarunee Prasertsopon; Chompunuch Klinmalai; Sunchai Payungporn; Anek Mungaomklang; Kulkanya Chokepha...; Rome Buathong; Arunee Thitithany...; Pilaipan Puthavat...
• Keywords: Enterovirus 71; Recombinant virus; Molecular Epidemiology; Coxsackievirus

Hand, foot, and mouth disease (HFMD) caused by enteroviruses remains a public health threat, particularly in the Asia-Pacific region during past two decades. Moreover, introduction of multiple subgenotypes emergence recombinant viruses is epidemiological importance. Based on either full genome or VP1 sequences, 32 (30 from HFMD patients, 1 an encephalitic patient, asymptomatic contact case) isolated Thailand between 2006 2014 were identified as 25 enterovirus 71 (EV71) isolates (comprising 20 B5, C2, 2 C4a, C4b subgenotypes) 7 coxsackievirus A16 (CA16) 6 B1a B1b subgenotypes). The EV71 subgenotype was introduced into for first time replaced C4a strains 2009. Phylogenetic, similarity plot bootscan analyses complete viral genomes 12 among isolates. Only one EV71-B5 isolate out virus with intratypic intertypic recombination, while all four EV71-C4 having undergone double seven CA16 viruses. recombination breakpoints these recombinants are located solely within P2 P3 regions. Surveillance circulating replacement important respect to molecular epidemiology selection upcoming vaccine. In addition, clinical importance needs be further explored.