Comparative metabolic profiling of Vitis amurensis and Vitis vinifera during cold acclimation

• DOI: 10.1038/s41438-018-0083-5
• Publication Date: 2018-12-31T03:35:06Z
• Authors: Fengmei Chai; Wenwen Liu; Yue Xiang; Xianbin Meng; Xiaoming Sun; Cheng Cheng; Guotian Liu; Lixin Duan; Haiping Xin; Shaohua Li
• Keywords: Stachyose; Citrus unshiu

Vitis amurensis is a wild plant that can withstand extreme cold temperatures. However, the accumulation of metabolites during acclimation (CA) in V. remains largely unknown. In this study, plantlets and vinifera cv. Muscat Hamburg were treated at 4 °C for 24 72 h, changes leaves detected by gas chromatography coupled with time-of-flight mass spectrometry. Most identified metabolites, including carbohydrates, amino acids, organic accumulated two types grape after CA. Galactinol, raffinose, fructose, mannose, glycine, ascorbate continuously induced amurensis, but not Hamburg. Twelve isoleucine, valine, proline, 2-oxoglutarate, putrescine, increased More galactinol, ascorbate, Hamburg, CA, which may be responsible excellent tolerance amurensis. The expression levels genes encoding β-amylase (BAMY), galactinol synthase (GolS), raffinose (RafS) evaluated quantitative reverse transcription-PCR. BAMY (VIT_02s0012 g00170) RafS (VIT_05s0077 g00840) primarily maltose respectively. was attributed to different members GolS grapes. conclusion, these results show inherent differences between under