Comparative transcriptomic analysis highlights contrasting levels of resistance of Vitis vinifera and Vitis amurensis to Botrytis cinerea

• DOI: 10.1038/s41438-021-00537-8
• Publication Date: 2021-05-03T11:48:24Z
• Authors: Ran Wan; Chunlei Guo; Xiaoqing Hou; Yanxun Zhu; Min Gao; Xiaoyan Hu; Songlin Zhang; Chen Jiao; Rongrong Guo; Zhi Li; Xiping Wang
• Keywords: 0303 health sciences; 03 medical and health sciences; Article

AbstractBotrytis cinereais a major grapevine (Vitisspp.) pathogen, but some genotypes differ in their degree of resistance. For example, theVitis viniferacultivar Red Globe (RG) is highly susceptible, butV. amurensisRupr Shuangyou (SY) is highly resistant. Here, we used RNA sequencing analysis to characterize the transcriptome responses of these two genotypes toB. cinereainoculation at an early infection stage. Approximately a quarter of the genes in RG presented significant changes in transcript levels during infection, the number of which was greater than that in the SY leaves. The genes differentially expressed between infected leaves of SY and RG included those associated with cell surface structure, oxidation, cell death and C/N metabolism. We found evidence that an imbalance in the levels of reactive oxygen species (ROS) and redox homeostasis probably contributed to the susceptibility of RG toB. cinerea. SY leaves had strong antioxidant capacities and improved ROS homeostasis following infection. Regulatory network prediction suggested that WRKY and MYB transcription factors are associated with the abscisic acid pathway. Weighted gene correlation network analysis highlighted preinfection features of SY that might contribute to its increased resistance. Moreover, overexpression ofVaWRKY10inArabidopsis thalianaandV. viniferaThompson Seedless enhanced resistance toB. cinerea. Collectively, our study provides a high-resolution view of the transcriptional changes of grapevine in response toB. cinereainfection and novel insights into the underlying resistance mechanisms.