Accurate immune repertoire sequencing reveals malaria infection driven antibody lineage diversification in young children

• DOI: 10.1038/s41467-017-00645-x
• Publication Date: 2017-09-11T10:13:04Z
• Authors: Ben S. Wendel; Chenfeng He; Mingjuan Qu; Di Wu; Stefany M. Hernandez; Ke-Yue Ma; Eugene W. Liu; Jun Xiao; Peter D. Crompton; Susan K. Pierce; Pengyu Ren; Keke Chen; Ning Jiang
• Keywords: Antibody Repertoire; Lineage (genetic); Hypogammaglobulinemia

Accurately measuring antibody repertoire sequence composition in a small amount of blood is challenging yet important for understanding responses to infection and vaccination. We develop molecular identifier clustering-based immune sequencing (MIDCIRS) use it study age-related development diversification before during acute malaria infants (< 12 months old) toddlers (12-47 with 4-8 ml blood. Here, we show this accurate high-coverage repertoire-sequencing method can as few 1000 naive B cells. Unexpectedly, discover high levels somatic hypermutation young 3 old. Antibody clonal lineage analysis reveals that are increased both upon infection, memory cells isolated from individuals who previously experienced continue induce hypermutations rechallenge. These results highlight the potential toddlers.Somatic antibodies occur but difficult track. Here authors present new called MIDCIRS deep quantitative cells, expand complexity response infection.