Construction of developmental lineage relationships in the mouse mammary gland by single-cell RNA profiling

• DOI: 10.1038/s41467-017-01560-x
• Publication Date: 2017-11-14T21:16:51Z
• Authors: Bhupinder Pal; Yunshun Chen; François Vaillant; Paul Jamieson; Lavinia Gordon; Anne C. Rios; Stephen Wilcox; Naiyang Fu; Kevin He Liu; Felicity C. Jackling; Melissa J. Davis; Geoffrey J. Lindeman; Gordon K. Smyth; Jane E. Visvader
• Keywords: Profiling (computer programming); Lineage (genetic); Cell lineage

The mammary epithelium comprises two primary cellular lineages, but the degree of heterogeneity within these compartments and their lineage relationships during development remain an open question. Here we report single-cell RNA profiling mouse epithelial cells spanning four developmental stages in post-natal gland. Notably, undergoes a large-scale shift gene expression from relatively homogeneous basal-like program pre-puberty to distinct lineage-restricted programs puberty. Interrogation transcriptomes reveals different levels diversity luminal basal compartments, identifies early progenitor subset marked by CD55. Moreover, uncover transit population rare mixed-lineage cluster amongst adult Together findings point hierarchy which prevails gland prior specification signatures, presence intermediates that may serve as or lineage-primed cells.