L-SCRaMbLE as a tool for light-controlled Cre-mediated recombination in yeast

• DOI: 10.1038/s41467-017-02208-6
• Publication Date: 2018-05-10T09:17:52Z
• Authors: Lena Hochrein; Leslie A. Mitchell; Karina Schulz; Katrin Messerschmidt; Bernd Mueller-Roeber
• Keywords: FLP-FRT recombination; Cre recombinase; Genome Engineering; Site-specific recombination

Abstract The synthetic yeast genome constructed by the International Synthetic Yeast Sc2.0 consortium adds thousands of loxPsym recombination sites to all 16 redesigned chromosomes, allowing shuffling chromosome parts Cre-loxP system thereby enabling evolution experiments. Here, we present L-SCRaMbLE, a light-controlled Cre recombinase for use in Saccharomyces cerevisiae . L-SCRaMbLE allows tight regulation activity with up 179-fold induction upon exposure red light. extent depends on time and concentration chromophore phycocyanobilin (PCB), which can be easily adjusted. tool presented here provides improved control over previously reported estradiol-dependent SCRaMbLE system, mediating larger variety possible events SCRaMbLE-ing reporter plasmid. Thereby, boosts potential further customization facile application S. re-engineering project or other recombination-based systems.