Direct cell–cell contact between mature osteoblasts and osteoclasts dynamically controls their functions in vivo

• DOI: 10.1038/s41467-017-02541-w
• Publication Date: 2018-01-15T11:07:32Z
• Authors: Masayuki Furuya; Junichi Kikuta; Sayumi Fujimori; Shigeto Seno; Hiroki Maeda; Mai Shirazaki; Maki Uenaka; Hiroki Mizuno; Yoriko Iwamoto; Akito Morimoto; Kunihiko Hashimoto; Takeshi Ito; Yukihiro Isogai; Masafumi Kashii; Takashi Kaito; Shinsuke Ohba; Ung-il Chung; Alexander C. Lich...; Kazuya Kikuchi; Hideo Matsuda; Hideki Yoshikawa; Masaru Ishii
• Keywords: Bone cell; Homeostasis

Bone homeostasis is regulated by communication between bone-forming mature osteoblasts (mOBs) and bone-resorptive osteoclasts (mOCs). However, the spatial-temporal relationship mode of interaction in vivo remain elusive. Here we show, using an intravital imaging technique, that mOB mOC functions are via direct cell-cell contact these cell types. The mOBs mOCs mainly occupy discrete territories steady state, although detected spatiotemporally limited areas. In addition, a pH-sensing fluorescence probe reveals secrete protons for bone resorption when they not with mOBs, whereas contacting non-resorptive, suggesting can inhibit contact. Intermittent administration parathyroid hormone causes anabolic effects, which lead to mixed distribution mOCs, increase This study spatiotemporal intercellular interactions affecting vivo.