Drosha drives the formation of DNA:RNA hybrids around DNA break sites to facilitate DNA repair

• DOI: 10.1038/s41467-018-02893-x
• Publication Date: 2018-02-01T10:39:56Z
• Authors: Wei-Ting Lu; Ben R. Hawley; George L. Skalka; Robert A. Baldock; Ewan M. Smith; Aldo S. Bader; Michal Malewicz; Felicity Z. Watts; Ania Wilczynska; Martin Bushell
• Keywords: Drosha; Dicer

Abstract The error-free and efficient repair of DNA double-stranded breaks (DSBs) is extremely important for cell survival. RNA has been implicated in the resolution damage but mechanism remains poorly understood. Here, we show that miRNA biogenesis enzymes, Drosha Dicer, control recruitment factors from multiple pathways to sites damage. Depletion significantly reduces by both homologous recombination (HR) non-homologous end joining (NHEJ). required within minutes break induction, suggesting a central early role processing repair. Sequencing DNA:RNA hybrids reveals invasion around Drosha-dependent manner. Removal component these structures results impaired These how can be direct critical mediator human cells.