Resolving molecule-specific information in dynamic lipid membrane processes with multi-resonant infrared metasurfaces
Science
Circular Dichroism
Q
Lipid Bilayers
Membrane Proteins
Reproducibility of Results
Biosensing Techniques
02 engineering and technology
01 natural sciences
Article
0104 chemical sciences
Membrane Lipids
Spectroscopy, Fourier Transform Infrared
Peptides
0210 nano-technology
Protein Binding
DOI:
10.1038/s41467-018-04594-x
Publication Date:
2018-05-29T12:02:50Z
AUTHORS (11)
ABSTRACT
AbstractA multitude of biological processes are enabled by complex interactions between lipid membranes and proteins. To understand such dynamic processes, it is crucial to differentiate the constituent biomolecular species and track their individual time evolution without invasive labels. Here, we present a label-free mid-infrared biosensor capable of distinguishing multiple analytes in heterogeneous biological samples with high sensitivity. Our technology leverages a multi-resonant metasurface to simultaneously enhance the different vibrational fingerprints of multiple biomolecules. By providing up to 1000-fold near-field intensity enhancement over both amide and methylene bands, our sensor resolves the interactions of lipid membranes with different polypeptides in real time. Significantly, we demonstrate that our label-free chemically specific sensor can analyze peptide-induced neurotransmitter cargo release from synaptic vesicle mimics. Our sensor opens up exciting possibilities for gaining new insights into biological processes such as signaling or transport in basic research as well as provides a valuable toolkit for bioanalytical and pharmaceutical applications.
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