Human in vivo-generated monocyte-derived dendritic cells and macrophages cross-present antigens through a vacuolar pathway

• DOI: 10.1038/s41467-018-04985-0
• Publication Date: 2018-06-26T16:18:32Z
• Authors: Tsing-Lee Tang-Huau; Paul Gueguen; Christel Goudot; Mélanie Durand; Mylène Bohec; Sylvain Baulande; Benoit Pasquier; Sebastian Amigorena; Elodie Segura
• Keywords: Cross-Presentation; Monocyte

Presentation of exogenous antigens on MHC-I molecules, termed cross-presentation, is essential for cytotoxic CD8+ T cell responses. In mice, dendritic cells (DCs) that arise from monocytes (mo-DCs) during inflammation have a key function in these responses by cross-presenting locally peripheral tissues. Whether human naturally-occurring mo-DCs can cross-present unknown. Here, we use and macrophages directly purified ascites to address this question. Single-cell RNA-seq data show CD1c+ DCs contain exclusively monocyte-derived cells. Both efficiently, but are inefficient transferring proteins into their cytosol. Inhibition cysteine proteases, not proteasome, abolishes cross-presentation We conclude using vacuolar pathway. Finally, only provide co-stimulatory signals induce effector Our findings thus important insights how harness therapeutic purposes.