Antibiotic-induced microbiome depletion alters metabolic homeostasis by affecting gut signaling and colonic metabolism

• DOI: 10.1038/s41467-018-05336-9
• Publication Date: 2018-07-16T16:17:37Z
• Authors: Amir Zarrinpar; Amandine Chaix; Zhenjiang Z. Xu; Max W. Chang; Clarisse A. Marotz; Alan Saghatelian; Rob Knight; Satchidananda Panda
• Keywords: Homeostasis; Carbohydrate Metabolism; Energy homeostasis; Short-chain fatty acid

Abstract Antibiotic-induced microbiome depletion (AIMD) has been used frequently to study the role of gut in pathological conditions. However, unlike germ-free mice, effects AIMD on host metabolism remain incompletely understood. Here we show elucidate its homeostasis, luminal signaling, and metabolism. We demonstrate that AIMD, which decreases Firmicutes Bacteroidetes species, baseline serum glucose levels, reduces surge a tolerance test, improves insulin sensitivity without altering adiposity. These changes occur setting decreased short-chain fatty acids (SCFAs), especially butyrate, secondary bile acid pool, affects whole-body In alters cecal gene expression glucagon-like peptide 1 signaling. Extensive tissue remodeling availability SCFAs shift colonocyte toward utilization. suggest homeostasis by potentially shifting energy utilization from glucose.