Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing

• DOI: 10.1038/s41467-018-05425-9
• Publication Date: 2018-07-26T10:35:53Z
• Authors: Duverney Chaverra...; Vanessa M. Macias; Grant L. Hughes; Sujit Pujhari; Yasutsugu Suzuki; David R. Peterson; Donghun Kim; Sage McKeand; Jason L. Rasgon
• Keywords: Gene drive; Transduction (biophysics)

Abstract Cas9-mediated gene editing is a powerful tool for addressing research questions in arthropods. Current approaches rely upon delivering Cas9 ribonucleoprotein (RNP) complex by embryonic microinjection, which challenging, limited to small number of species, and inefficient even optimized taxa. Here we develop technology termed Receptor-Mediated Ovary Transduction Cargo (ReMOT Control) deliver RNP the arthropod germline injection into adult female mosquitoes. We identify peptide (P2C) that mediates transduction from hemolymph developing mosquito oocytes, resulting heritable offspring with efficiency as high 0.3 mutants per injected mosquito. demonstrate P2C functions six species. Identification taxa-specific ovary-specific ligand–receptor pairs may further extend use ReMOT Control novel