Visual and modular detection of pathogen nucleic acids with enzyme–DNA molecular complexes

• DOI: 10.1038/s41467-018-05733-0
• Publication Date: 2018-08-07T18:13:27Z
• Authors: Nicholas R. Y. Ho; Geok Soon Lim; Noah R. Sundah; Diana Lim; Tze Ping Loh; Huilin Shao
• Keywords: Pathogen-associated molecular pattern

Abstract Rapid, visual detection of pathogen nucleic acids has broad applications in infection management. Here we present a modular platform, termed enzyme-assisted nanocomplexes for identification (enVision). The system consists an integrated circuit enzyme–DNA nanostructures, which function as independent recognition and signaling elements, direct versatile from infected cells. built-in enzymatic cascades produce rapid color readout the naked eye; assay is thus fast (<2 h), sensitive (<10 amol), readily quantified with smartphones. When implemented on configurable microfluidic technology demonstrates superior programmability to perform computations, detecting diverse targets their virus–host genome integration loci. We further design enVision platform molecular-typing infections patient endocervical samples. not only improves clinical inter-subtype differentiation, but also expands intra-subtype coverage identify previously undetectable infections.