Cell-specific proteome analyses of human bone marrow reveal molecular features of age-dependent functional decline

• DOI: 10.1038/s41467-018-06353-4
• Publication Date: 2018-09-25T09:19:59Z
• Authors: Marco L. Hennrich; Natalie Romanov; Patrick Horn; Samira Jaeger; Volker Eckstein; Violetta Steeples; Fei Ye; Ximing Ding; Laura Poisa-Beiro; Mang Ching Lai; Benjamin Lang; Jacqueline Boultwood; Thomas Luft; Judith B. Zaugg; Andrea Pellagatti; Peer Bork; Patrick Aloy; Anne-Claude Gavin; Anthony D. Ho
• Keywords: Proteome; Homing (biology)

Abstract Diminishing potential to replace damaged tissues is a hallmark for ageing of somatic stem cells, but the mechanisms remain elusive. Here, we present proteome-wide atlases age-associated alterations in human haematopoietic and progenitor cells (HPCs) five other cell populations that constitute bone marrow niche. For each, abundance large fraction ~12,000 proteins identified assessed 59 subjects from different ages. As HPCs become older, pathways central carbon metabolism exhibit features reminiscent Warburg effect, where glycolytic intermediates are rerouted towards anabolism. Simultaneously, altered early regulators HPC differentiation reveals reduced functionality bias myeloid differentiation. Ageing causes niche too, diminishes involved homing. The data represent valuable resource further analyses, validation knowledge gained animal models.