Robust single-cell DNA methylome profiling with snmC-seq2

• DOI: 10.1038/s41467-018-06355-2
• Publication Date: 2018-09-14T09:51:41Z
• Authors: Chongyuan Luo; Angeline Rivkin; Jingtian Zhou; Justin P. Sandoval; Laurie Kurihara; Jacinta Lucero; Rosa Castanon; Joseph R. Nery; António Pinto-Duarte; Brian Bui; Conor Fitzpatrick; Carolyn O’Connor; Seth Ruga; Marc E. Van Eden; David A. Davis; Deborah C. Mash; M. Margarita Behrens; Joseph R. Ecker
• Keywords: Epigenomics; Reprogramming; Profiling (computer programming)

Abstract Single-cell DNA methylome profiling has enabled the study of epigenomic heterogeneity in complex tissues and during cellular reprogramming. However, broader applications method have been impeded by modest quality sequencing libraries. Here we report snmC-seq2, which provides improved read mapping, reduced artifactual reads, enhanced throughput, as well increased library complexity coverage uniformity compared to snmC-seq. snmC-seq2 is an efficient strategy suited for large-scale single-cell studies.