Long non-coding RNA-dependent mechanism to regulate heme biosynthesis and erythrocyte development

• DOI: 10.1038/s41467-018-06883-x
• Publication Date: 2018-10-16T12:14:13Z
• Authors: Jinhua Liu; Yapu Li; Jingyuan Tong; Jie Gao; Qing Guo; Lingling Zhang; Bingrui Wang; Hui Zhao; Hongtao Wang; Erlie Jiang; Ryo Kurita; Yukio Nakamura; Osamu Tanabe; James Douglas Engel; Emery H. Bresnick; Jiaxi Zhou; Lihong Shi
• Keywords: Circular RNA

In addition to serving as a prosthetic group for enzymes and hemoglobin structural component, heme is crucial homeostatic regulator of erythroid cell development function. While lncRNAs modulate diverse physiological pathological cellular processes, their involvement in heme-dependent mechanisms largely unexplored. this study, we elucidated lncRNA (UCA1)-mediated mechanism that regulates metabolism human cells. We discovered UCA1 expression dynamically regulated during maturation, with maximal proerythroblasts. depletion predominantly impairs biosynthesis arrests differentiation at the proerythroblast stage. Mechanistic analysis revealed physically interacts RNA-binding protein PTBP1, functions an RNA scaffold recruit PTBP1 ALAS2 mRNA, which stabilizes mRNA. These results define lncRNA-mediated posttranscriptional provides new dimension into how fundamental biosynthetic process determinant erythrocyte development.