A linear nonribosomal octapeptide from Fusarium graminearum facilitates cell-to-cell invasion of wheat

• DOI: 10.1038/s41467-019-08726-9
• Publication Date: 2019-02-25T11:03:08Z
• Authors: Lei-Jie Jia; Hao-Yu Tang; Wan-Qiu Wang; Ting-Lu Yuan; Wan-Qian Wei; Bo Pang; Xue-Min Gong; Shou-Feng Wang; Yu-Jie Li; Dong Zhang; Wen Liu; Wei-Hua Tang
• Keywords: Coleoptile; Nonribosomal peptide

Abstract Fusarium graminearum is a destructive wheat pathogen. No fully resistant cultivars are available. Knowledge concerning the molecular weapons of F. to achieve infection remains limited. Here, we report that deletion putative secondary metabolite biosynthesis gene cluster fg3_54 compromises pathogen’s ability infect through cell-to-cell penetration. Ectopic expression fgm4 , pathway-specific bANK-like regulatory gene, activates transcription in vitro. We identify linear, C- terminally reduced and d -amino acid residue-rich octapeptide, fusaoctaxin A, as product two nonribosomal peptide synthetases encoded by . Chemically-synthesized A restores invasiveness -deleted enables colonization coleoptiles strains lack homolog nonpathogenic wheat. In conclusion, our results virulence factor required for invasion