Large-scale lipid analysis with C=C location and sn-position isomer resolving power

• DOI: 10.1038/s41467-019-14180-4
• Publication Date: 2020-01-17T11:03:05Z
• Authors: Wenbo Cao; Simin Cheng; Jing Yang; Jiaxin Feng; Wenpeng Zhang; Zishuai Li; Qinhua Chen; Yu Xia; Zheng Ouyang; Xiaoxiao Ma
• Keywords: Position (finance)

Lipids play a pivotal role in biological processes and lipid analysis by mass spectrometry (MS) has significantly advanced lipidomic studies. While the structure specificity of proves to be critical for studying functions lipids, current mainstream methods large-scale can only identify classes fatty acyl chains, leaving C=C location sn-position unidentified. In this study, combining photochemistry tandem MS we develop simple but effective workflow enable near-complete characterization with powerful capability identifying location(s) sn-position(s) simultaneously. Quantitation isomers at multiple levels is achieved different subtypes human breast cancer cells are successfully discriminated. Remarkably, lung tissues distinguished from adjacent normal using quantitative results both isomers.