Super-resolution microscopy compatible fluorescent probes reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics

• DOI: 10.1038/s41467-020-14309-w
• Publication Date: 2020-01-24T11:03:06Z
• Authors: Julia Ast; Anastasia Arvaniti; Nicholas H. F. Fine; Daniela Nasteska; Fiona B. Ashford; Zania Stamataki; Zsombor Koszegi; Andrea Bacon; Ben J. Jones; Maria A. Lucey; Shugo Sasaki; Daniel I. Brierley; Benoit Hastoy; Alejandra Tomas; Giuseppe D’Agostino; Frank Reimann; Francis C. Lynn; Christopher A. Re...; Amelia K. Linnemann; Elisa D’Este; Davide Calebiro; Stefan Trapp; Kai Johnsson; Tom Podewin; Johannes Broichhagen; David J. Hodson
• Keywords: Dynamics

Abstract The glucagon-like peptide-1 receptor (GLP1R) is a class B G protein-coupled (GPCR) involved in metabolism. Presently, its visualization limited to genetic manipulation, antibody detection or the use of probes that stimulate activation. Herein, we present LUXendin645 , far-red fluorescent GLP1R antagonistic peptide label. produces intense and specific membrane labeling throughout live fixed tissue. signaling can additionally be evoked when allosterically modulated presence . Using LUXendin651 describe islet, brain hESC-derived β-like cell expression patterns, reveal higher-order organization including nanodomains, track single subpopulations. We furthermore show LUXendin backbone optimized for intravital two-photon imaging by installing red fluorophore. Thus, our super-resolution compatible allow endogenous GLP1R, provide insight into GPCR distribution dynamics both vitro vivo.