Versatile whole-organ/body staining and imaging based on electrolyte-gel properties of biological tissues

• DOI: 10.1038/s41467-020-15906-5
• Publication Date: 2020-04-27T10:03:17Z
• Authors: Etsuo A. Susaki; Chika Shimizu; Akihiro Kuno; Kazuki Tainaka; Xiang Li; Kengo Nishi; Ken Morishima; Hiroaki Ono; Koji L. Ode; Yuki Saeki; Kazunari Miyamichi; Kaoru Isa; Chihiro Yokoyama; Hiroki Kitaura; Masako Ikemura; Tetsuo Ushiku; Yoshihiro Shimizu; Takashi Saito; Takaomi C. Saido; Masashi Fukayama; Hirotaka Onoe; Kazushige Touhara; Tadashi Isa; Akiyoshi Kakita; Mitsuhiro Shibayama; Hiroki R. Ueda
• Keywords: Adult; Male; 570; Science; 610; Article; Electrolytes; Mice; 03 medical and health sciences; Imaging, Three-Dimensional; Cerebellum; Image Processing, Computer-Assisted; Animals; Humans; Fluorescent Dyes; 0303 health sciences; Q; Optical Imaging; Brain; Callithrix; 3. Good health; Mice, Inbred C57BL; Animals, Newborn; Microscopy, Fluorescence; Female

Abstract Whole-organ/body three-dimensional (3D) staining and imaging have been enduring challenges in histology. By dissecting the complex physicochemical environment of system, we developed a highly optimized 3D pipeline based on CUBIC. Based our precise characterization biological tissues as an electrolyte gel, experimentally evaluated broad conditions by using artificial tissue-mimicking material. The combination allows bottom-up design superior protocol that can uniformly label whole adult mouse brains, marmoset brain hemisphere, ~1 cm 3 tissue block postmortem human cerebellum, entire infant body with dozens antibodies cell-impermeant nuclear stains. whole-organ images collected light-sheet microscopy are used for computational analyses comparison analysis between species. This pipeline, named CUBIC-HistoVIsion, thus offers advanced opportunities organ- organism-scale histological multicellular systems.