Plasma-derived extracellular vesicles from Plasmodium vivax patients signal spleen fibroblasts via NF-kB facilitating parasite cytoadherence

• DOI: 10.1038/s41467-020-16337-y
• Publication Date: 2020-06-02T10:05:50Z
• Authors: Haruka Toda; Miriam Diaz-Varela; Joan Segui-Barber; Wanlapa Roobsoong; Barbara Baro; Susana Garcia-Silva; Alicia Galiano; Melisa Gualdrón-L...; Anne C. G. Almeida; Marcelo A. M. Brito; Gisely Cardoso de...; Iris Aparici-Herraiz; Carlos Castro-Cav...; Wuelton Marcelo M...; Eva Borràs; Eduard Sabidó; Igor C. Almeida; Jakub Chojnacki; Javier Martinez-P...; Maria Calvo; Pilar Armengol; Jaime Carmona-Fon...; Maria Fernanda Ya...; Ricardo Lauzurica; Antonio Marcilla; Hector Peinado; Mary R. Galinski; Marcus V. G. Lacerda; Jetsumon Sattabon...; Carmen Fernandez-...; Hernando A. del P...
• Keywords: Extracellular Vesicles

Abstract Plasmodium vivax is the most widely distributed human malaria parasite. Previous studies have shown that circulating microparticles during P. acute attacks are indirectly associated with severity. Extracellular vesicles (EVs) therefore major components of plasma holding insights into pathological processes. Here, we demonstrate plasma-derived EVs from patients ( Pv EVs) preferentially uptaken by spleen fibroblasts h SFs) as compared to uptake healthy individuals. Moreover, this induces specific upregulation ICAM-1 translocation NF-kB nucleus. After uptake, -infected reticulocytes obtained show adhesion properties SFs, reversed inhibiting Together, these data provide physiological EV-based mechanisms pathology and support existence -adherent parasite subpopulations in microvasculature spleen.